Amino terminal fusion of heterologous proteins to CotC increases display efficiencies in the Bacillus subtilis spore system.
نویسندگان
چکیده
منابع مشابه
A system of vectors for Bacillus subtilis spore surface display
BACKGROUND Bacterial spores have been utilized as platforms for protein display. The best studied display systems are based on Bacillus subtilis spores in which several coat proteins have successfully been used as anchors for heterologous protein. Increasing knowledge about spore coat structure enables selection of new anchor proteins such as CotZ and CgeA. Here we describe a system of vectors ...
متن کاملExpression and display of UreA of Helicobacter acinonychis on the surface of Bacillus subtilis spores
BACKGROUND The bacterial endospore (spore) has recently been proposed as a new surface display system. Antigens and enzymes have been successfully exposed on the surface layers of the Bacillus subtilis spore, but only in a few cases the efficiency of expression and the effective surface display and have been determined. We used this heterologous expression system to produce the A subunit of the...
متن کاملThe choice of the anchoring protein influences the interaction of recombinant Bacillus spores with the immune system.
The technology of display of heterologous proteins on the surface of Bacillus subtilis spores enables use of these structures as carriers of antigens for mucosal vaccination. Currently, there are no technical possibilities to predict whether a designed fusion will be efficiently displayed on the spore surface and how such recombinant spores will interact with cells of the immune system. In this...
متن کاملCotC-CotU heterodimerization during assembly of the Bacillus subtilis spore coat.
We report evidence that CotC and CotU, two previously identified components of the Bacillus subtilis spore coat, are produced concurrently in the mother cell chamber of the sporulating cell under the control of sigmaK and GerE and immediately assembled around the forming spore. In the coat, the two proteins interact to form a coat component of 23 kDa. The CotU-CotC interaction was not detected ...
متن کاملSurface display of recombinant proteins on Bacillus subtilis spores.
We developed a novel surface display system based on the use of bacterial spores. A protein of the Bacillus subtilis spore coat, CotB, was found to be located on the spore surface and used as fusion partner to express the 459-amino-acid C-terminal fragment of the tetanus toxin (TTFC). Western, dot blot and fluorescent-activated cell sorting analyses were used to monitor TTFC surface expression ...
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ورودعنوان ژورنال:
- BioTechniques
دوره 42 2 شماره
صفحات -
تاریخ انتشار 2007